Cloning and Expression of VP2 Gene of Infectious Bursal Disease Virus in Eukaryotic Cells

نویسندگان

  • Hamid Galehdari Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, P.O. Box 65355-141, Ahvaz, I.R. Iran
  • Masoud Reza Seyfiabad Shapouri Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, P.O. Box 65355-145, Ahvaz, I.R. Iran
  • Mohammad Roayaei Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, P.O. Box 65355-141, Ahvaz, I.R. Iran
  • Roozbeh Hushiarian Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, P.O. Box 65355-141, Ahvaz, I.R. Iran
چکیده مقاله:

Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. This region of 1356 bp was inserted into a eukaryotic expression plasmid, pCDNA4, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Plasmid DNA was transfected into COS-7 cell line and transient expression of VP2 from the constructed plasmid was characterized by dot blotting with a polyclonal antibody to IBDV.

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Cloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells

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cloning and secretory expression of vp2 gene of infectious bursal disease virus in eukaryotic cells

vp2 gene coding region of a vaccinal strain (d78) of infectious bursal disease virus (ibdv) was clonedin a eukaryotic expression vector, psec tag2a. the gene was placed downstream of ig κ chain leadersequence, under the control of human cytomegalovirus (hcmv) immediate early enhancer and promoter. theconstruct psec tag2a-vp2 was transfected in cos-7 cell line and the expression and secretion of...

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cloning and expression of vp2 gene of infectious bursal disease virus in eukaryotic cells

infectious bursal disease (ibd) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. vp2 is the major host-protective protein of infectious bursal disease virus (ibdv). the encoding region of vp2 protein was pcr amplified from a plasmid containing a cdna fragment of large genomic segment of ibdv, strain d78. thi...

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Cloning and expression of VP2 gene of Infectious bursal disease virus in eukaryotic cells

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Cloning and Codon-optimized Expression of Structural Protein Hypervariable Region of VP2 from Infectious Bursal Disease Virus

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عنوان ژورنال

دوره 5  شماره 4

صفحات  246- 250

تاریخ انتشار 2007-10-01

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